CST, 3410T, Drosha (D30F3) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Drosha. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Drosha (D30F3) Rabbit Monoclonal Antibody (CST #3410) is ready to ship. Product Usage Information For optimal ChIP results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:25 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity Drosha (D30F3) Rabbit Monoclonal Antibody detects endogenous levels of total Drosha protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His549 of human Drosha. Background Drosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components, such as DGCR8 (2). Drosha and DGCR8 are necessary for miRNA biogenesis (3). Alternate Names Drosha; drosha ribonuclease III; drosha, double-stranded RNA-specific endoribonuclease; drosha, ribonuclease type III; ETOHI2; HSA242976; nuclear RNase III Drosha; p241; Protein Drosha; putative protein p241 which interacts with transcription factor Sp1; putative ribonuclease III; RANSE3L; Ribonuclease 3; Ribonuclease III; ribonuclease III, nuclear; ribonuclease type III, nuclear; RN3; RNase III; RNASE3L; RNASEN; RNC Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG