CST, 34547S, CBX7 (E7N1W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CBX7. Validated for Western Blotting,Immunohistochemistry (Paraffin),Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, CBX7 (E7N1W) Rabbit Monoclonal Antibody (CST #34547) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 - 1:1600 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Chromatin IP Specificity / Sensitivity CBX7 (E7N1W) Rabbit Monoclonal Antibody recognizes endogenous levels of total CBX7 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CBX7 protein. Background The polycomb group (PcG) proteins contribute to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage Specification, cell death, and cell-cycle arrest (1-4). PcG proteins exist in two complexes that cooperate to maintain long-term gene silencing through epigenetic chromatin modifications. The first complex, EED-EZH2, is recruited to genes by DNA-binding transcription factors and methylates histone H3 on Lys27. This histone methyltransferase activity requires the Ezh2, Eed, and Suz12 subunits of the complex (5). Histone H3 methylation at Lys27 facilitates the recruitment of the second complex, PRC1, which ubiquitinylates histone H2A on Lys119 (6). Chromobox protein homolog 7 (CBX7) is a component of the PRC1 complex that binds to the H3K27me3 mark through its N-terminal chromodomain to facilitate transcriptional repression (7). CBX7 is believed to promote stem cell self-renewal via repression of differentiation-specific genes, in contrast to other CBX proteins that promote lineage commitment (8-10). CBX7 also plays a complex role in cancer, being described as either a tumor suppressor or proto-oncogene, depending on the cellular context and tissue type (7). For instance, CBX7 has been reported as an oncogene in follicular lymphoma and gastric cancer through repression of the tumor suppressor locus (11,12). In contrast, CBX7 functions as a tumor suppressor by negatively regulating expression in human lung carcinoma and glioma (13,14). Decreased CBX7 levels have also been correlated with increased malignancy and poor prognosis in other cancer types, including thyroid, bladder, pancreatic, breast, and colon carcinomas, suggesting a potential use for CBX7 as a prognostic biomarker (15-20). Alternate Names CBX7; chromobox 7; chromobox homolog 7; Chromobox protein homolog 7 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG