CST, 34594T, 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TNFRSF9. Validated for WB,IP,IHC,IF,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit Monoclonal Antibody (CST #34594) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:250 - 1:1000 Flow Cytometry (Fixed/Permeabilized): 1:400 - 1:1600 Flow Cytometry (Live): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 58350 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity 4-1BB/CD137/TNFRSF9 (D2Z4Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total 4-1BB/CD137/TNFRSF9 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the extracellular domain of human 4-1BB/CD137/TNFRSF9 protein. Background TNFRSF9 is a member of the tumor necrosis factor receptor superfamily (1,2). It is also called 4-1BB or CD137 (1, 2). 4-1BB/CD137/TNFRSF9 is expressed in activated CD4+ and CD8+ T cells, natural killer cells and dendritic cells (2-5). The ligand 4-1BBL/CD137L/TNFSF9 on antigen presenting cells binds to 4-1BB/CD137/TNFRSF9 and costimulates the activation of T cells (5). The binding of agonistic antibodies to 4-1BB/CD137/TNFRSF9 also leads to costimulation for T cell activation (5). Studies have shown the effectiveness of targeting 4-1BB/CD137/TNFRSF9 by its agonistic antibodies in cancer immunotherapy (6). Alternate Names 4-1BB; 4-1BB ligand receptor; CD137; CD137 antigen; CDw137; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; MGC2172; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; T-cell antigen ILA; TNF receptor superfamily member 9; TNFRSF9; TNR9; Tumor necrosis factor receptor superfamily member 9; tumor necrosis factor receptor superfamily, member 9 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 32, 40 Source/Isotype: Rabbit IgG