CST, 34662S, Stat5b Antibody

Polyclonal Antibody for studying STAT5B. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Stat5b Antibody (CST #34662) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Stat5b Antibody recognizes endogenous levels of total Stat5b protein. This antibody does not cross-react with Stat5a protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Stat5b protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Stat5 is activated in response to a wide variety of ligands, including IL-2, GM-CSF, growth hormone, and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6). Alternate Names GHISID2; Signal transducer and activator of transcription 5B; STA5B; STAT5; STAT5B; transcription factor STAT5B Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 SOURCE: Rabbit