CST, 35073S, SRSF3 (E9U9C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SFRS3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SRSF3 (E9U9C) Rabbit Monoclonal Antibody (CST #35073) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SRSF3 (E9U9C) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRSF3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn89 of human SRSF3 protein. Background Serine/arginine-rich splicing factor 3 (SRSF3), also known as SRP20, is a member of the highly conserved family of SR proteins responsible for splicing regulation (1,2). SRSF3 promotes exon inclusion by enhancing the recognition of weak splice acceptor sites, a function that also regulates its own mRNA by including exon 4. This function is antagonized by another SR family member, SRSF1 (3). SRSF3 can be shuttled in and out of the cytoplasm, where it plays a role in mRNA export along with SRSF7 (3,4). SRSF3 and SRSF7 have been shown to exert opposing effects on 3'UTR length by recruiting various proteins upstream of polyadenylation sites (5,6). SRSF3 has been shown to be regulated by an epitranscriptomic mechanism, as the m6a-binding factor YTHDC1 recruits SRSF3 to promote exon inclusion, while blocking the binding of SRSF10 (7). SRSF3 has been identified as an oncogene, which through its autoregulation and alternative splicing of other proteins, can drive many different cancer types (8-12). Alternate Names epididymis secretory sperm binding protein; pre-mRNA splicing factor SRp20; Pre-mRNA-splicing factor SRP20; serine and arginine rich splicing factor 3; Serine/arginine-rich splicing factor 3; SFRS3; Splicing factor, arginine/serine-rich 3; splicing factor, arginine/serine-rich, 20-kD; SRP20; SRSF3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 20, 22 Source/Isotype: Rabbit IgG