CST, 3512S, Connexin 43 Antibody

Polyclonal Antibody for studying Connexin-43. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, Connexin 43 Antibody (CST #3512) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Frozen): 1:50 - 1:100 Immunofluorescence (Immunocytochemistry): 1:50 - 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Connexin 43 Antibody detects endogenous levels of total connexin 43. This antibody does not cross-react with other connexins. Species Reactivity: Human, Mouse, Rat, Monkey, Zebrafish Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human connexin 43 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (1,2). Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication (3). Src can interact with and phosphorylate Cx43 to alter gap junction communication (4,5). Alternate Names AVSD3; CMDR; connexin 43; Connexin-43; Cx43; CXA1; DFNB38; EKVP; EKVP3; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction protein alpha 1; gap junction protein, alpha 1, 43kDa; gap junction protein, alpha-like; GJA1; GJAL; HLHS1; HSS; ODDD; PPKCA Specification REACTIVITY: H M R Mk Z SENSITIVITY: Endogenous MW (kDa): 39, 41, 43, 44 SOURCE: Rabbit