CST, 35173SF, Brn2/POU3F2 (D2C1L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying POU3F2/Brn2. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Brn2/POU3F2 (D2C1L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #35173) is ready to ship. Product Usage Information This product is the carrier free version of product #12137. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12137 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Brn2/POU3F2 (D2C1L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Brn2/POU3F2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro97 of human Brn2/POU3F2 protein. Background Brn2/POU3F2 is a POU domain-containing transcription factor involved in neuronal differentiation and activation of the corticotrophin-releasing hormone gene (1,2). In mice, disruption of the Brn2 gene results in loss of specific neuronal lineages in the hypothalamus (3). In addition to its role in mammalian neurogenesis, Brn2 has also been implicated in melanoma tumorigenesis and has been shown in the literature to be overexpressed in human melanoma cells compared to normal melanocytes (4,5). Recent studies also identify Brn2 as a transcription factor playing an important role in keratinocyte differentiation (6). Recent reports demonstrate that overexpression of three transcription factors (Brn2, Ascl1, and Myt1L) can directly convert human fibroblasts into functional neurons under precisely defined conditions (7,8). Alternate Names Brain-2; Brain-specific homeobox/POU domain protein 2; Brn-2; BRN2; N-Oct3; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; OCT7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF-7; OTF7; PO3F2; POU class 3 homeobox 2; POU domain, class 3, transcription factor 2; POU3F2; POUF3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG