CST, 35176SF, Semaphorin-4A (E5N3K) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying SEMA4A. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Semaphorin-4A (E5N3K) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #35176) is ready to ship. Product Usage Information This product is the carrier free version of product #57331. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 57331 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Semaphorin-4A (E5N3K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Semaphorin-4A protein. An unknown background band is detected at 55 kDa in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu756 of human Semaphorin-4A protein. Background Semaphorin-4A, or Sema4A, is a membrane-type class IV semaphorin family member glycoprotein and is expressed in dendritic cells, B cells, T cells, and endothelial cells (1). The protein structure consists of an N-terminus signal peptide, a sema domain, a C2 type Ig domain, a transmembrane region, and a cytoplasmic tail (2). Semaphorin-4A exerts diverse biological effects through several different receptor-mediated pathways and plays important roles in immunity, angiogenesis, neuronal migration, and tumor progression. Semaphorin-4A enhances T cell activation by interacting with TIM-2, and T cell-derived Semaphorin-4A plays a role in helper T cell (Th) differentiation (3,4). In regulatory T cells (Tregs), Semaphorin-4A plays an important role in enhancing stability through interactions with neuropilin-1 (Nrp1) (5). Semaphorin-4A interacts with Plexin D1, which is expressed in endothelial cells and negatively regulates angiogenesis (6). In presynaptic neurons, Semaphorin-4A promotes the development of inhibitory or excitatory synapses through interactions with Plexin B1 or Plexin B2, respectively (7). Semaphorin-4A has been identified as a therapeutic target to promote antitumor responses, and its expression in the tumor microenvironment (TME) has been suggested as a biomarker to predict response to immunotherapy (8). Alternate Names CORD10; FLJ12287; RP35; SEM4A; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4A; SEMA4A; SEMAB; semaphorin 4A; semaphorin B; Semaphorin-4A; Semaphorin-B; SEMB Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG