CST, 46940T, PPT1 (F7C5U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PPT1 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, PPT1 (F7C5U) Rabbit Monoclonal Antibody (CST #46940) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity PPT1 (F7C5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total PPT1 protein. Mouse reactivity is determined by IHC. Nonspecific diffuse cytoplasmic staining was observed in normal human esophageal epithelium and cytoplasmic/nuclear staining was observed in mouse and human small intestine goblet cells. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly250 of mouse PPT1 protein. Background Palmitoyl-protein thioesterase 1 (PPT1) is a depalmitoylation enzyme that removes palmitate groups from S-acylated proteins (1). PPT1 localization has been demonstrated in multiple compartments, including membrane rafts, the nucleus, lysosomes, the Golgi apparatus, synaptic vesicles, axons, and the endoplasmic reticulum (ER) (1,2). Mutations of the PPT1 protein can cause neuronal ceroid lipofuscinosis (NCL) type 1 (CLN1), a lysosomal storage disorder characterized by brain atrophy, mental and motor retardation, seizures, and retinal degeneration, ultimately resulting in blindness (2,3). In healthy, aged brains, PPT1 and other CLN proteins have been identified as lipofuscin constituents (4). Alternate Names 9530043G02Rik; AA960502; C77813; CLN1; D4Ertd184; D4Ertd184e; INCL; MGC107420; OTTMUSP00000009253; Palmitoyl-protein hydrolase 1; Palmitoyl-protein thioesterase 1; Ppt; PPT-1; Ppt1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 29, 35 Source/Isotype: Rabbit IgG