CST, 46970S, TUT7 Antibody

Polyclonal Antibody for studying ZCCHC6. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, TUT7 Antibody (CST #46970) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TUT7 Antibody recognizes endogenous levels of total TUT7 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val741 of human TUT7 protein. Antibodies are purified by peptide affinity chromatography. Background Terminal uridylyl transferase 4 and 7 (TUT4/7), also known as ZCCHC11 and ZCCHC6 respectively, are enzymes that posttranscriptionally uridylate mRNAs and miRNAs at the 3' end (1,2). The TUT enzymes can monouridylate double-stranded RNA with a single nucleotide overhang, which in turn creates a recognition site for Dicer to process miRNAs (3). Many of the miRNAs recognized by TUT4/7 are involved in cell differentiation such as the gene cluster (4). TUT4 and TUT7 have been shown to be recruited by Lin28 in stem cells to uridylate and block expression of let-7 miRNA, a microRNA with tumor suppressive qualities (3,5,6). TUT4 and TUT7 play a role in the mRNA decay pathway, where they polyuridylate targeted mRNAs to promote turnover (7). TUT4 and TUT7 have also been implicated in T cell activation, innate immune response, and antiviral defenses (8-10). Both TUT proteins have also been shown to prevent retrotransposition of LINE-1 elements, indicating a role in maintaining genomic stability (11). Alternate Names DKFZp666B142; DKFZp686C11112; DKFZp686F119; DKFZp686I1269; HS2; KIAA1711; PAP associated domain containing 6; PAPD6; TENT3B; terminal uridylyl transferase 7; Terminal uridylyltransferase 7; TUT7; TUTase 7; ZCCHC6; Zinc finger CCHC domain-containing protein 6; zinc finger CCHC-type containing 6; zinc finger, CCHC domain containing 6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 205 SOURCE: Rabbit