CST, 47043T, Phospho-Glycogen Synthase (Ser641) (D4H1B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Glycogen Synthase (Ser641) phosphate. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Glycogen Synthase (Ser641) (D4H1B) Rabbit Monoclonal Antibody (CST #47043) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 98074 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-Glycogen Synthase (Ser641) (D4H1B) Rabbit Monoclonal Antibody recognizes endogenous levels of both muscle and liver isoforms of glycogen synthase protein only when phosphorylated at Ser641. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Ser641 of human liver glycogen synthase. Background Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver (1). Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals: muscle isoform (glycogen synthase 1, GYS1) and liver isoform (glycogen synthase 2, GYS2), respectively (1). Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649, and Ser653), inhibiting its activity (2,3). Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth (1). Alternate Names Glycogen [starch] synthase, muscle; glycogen synthase 1; glycogen synthase 1 (muscle); GSY; GYS; GYS1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 85-90 Source/Isotype: Rabbit IgG