CST, 51786T, UAP1 (F5K2U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UAP1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, UAP1 (F5K2U) Rabbit Monoclonal Antibody (CST #51786) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity UAP1 (F5K2U) Rabbit Monoclonal Antibody recognizes endogenous levels of total UAP1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UAP1 protein. Background UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1) is a metabolic enzyme that plays a key role in the hexosamine biosynthesis pathway (HBP). UAP1 catalyzes the final step in the pathway by converting uridine triphosphate (UTP) and GlcNAc-1-phosphate to UDP-GlcNAc, a nucleotide sugar (1). UDP-GlcNAc is further utilized as a substrate for multiple glycosylation reactions, including O-GlcNAcylation of target proteins (2). HBP dysfunction has been linked to various cancers, potentially due to elevated O-GlcNAcylation levels affecting signaling pathways (3). UAP1 expression levels are particularly elevated in prostate cancer, and knockdown of UAP1 has been found to reduce cell growth and viability in prostate cancer cells (4-6). Altered UAP1 expression levels have also been linked to other cancers, such as osteosarcoma and breast cancer (7,8). Alternate Names AGX; AGX-1; AGX-2; AGX1; AGX2; Antigen X; SPAG2; sperm associated antigen 2; Sperm-associated antigen 2; testis tissue sperm-binding protein Li 37a; UAP1; UDP-N-acetylgalactosamine pyrophosphorylase; UDP-N-acetylglucosamine diphosphorylase 1; UDP-N-acetylglucosamine pyrophosphorylase; UDP-N-acetylglucosamine pyrophosphorylase 1; UDP-N-acetylhexosamine pyrophosphorylase; UDP-N-acetylhexosamine pyrophosphorylase 1; UDP-N-acteylglucosamine pyrophosphorylase 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 62, 59 Source/Isotype: Rabbit IgG