CST, 5182S, TRIM29/ATDC Antibody

Polyclonal Antibody for studying ATDC/TRIM29. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, TRIM29/ATDC Antibody (CST #5182) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TRIM29/ATDC Antibody detects endogenous levels of TRIM29/ATDC protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu160 of human TRIM29/ATDC protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Tripartite motif-containing protein 29 (TRIM29, ATDC) was isolated as a candidate gene by its ability to complement the radiosensitivity defect of an ataxia-telangiectasia (AT) cell line (1). This putative transcription regulator belongs to the TRIM (tripartite motif) protein family that is characterized by highly conserved amino-terminal RING finger, B-box, and coiled-coil domains. The TRIM29 protein binds and sequesters cytosolic p53, repressing expression of p53 target genes including p21 and Noxa by preventing p53 from entering the nucleus. Expression of TRIM29 inhibits p53 function and results in increased cell proliferation. (2). TRIM29 enhances tumor growth and metastasis and high TRIM29 levels are seen in most invasive pancreatic cancers. The oncogenic effect of TRIM29 appears to require β-catenin as expression of both proteins is elevated in pancreatic cancer cell lines and tissues (3). Alternate Names Ataxia telangiectasia group D-associated protein; ataxia-telangiectasia group D-associated protein; ATDC; FLJ36085; TRI29; TRIM29; tripartite motif containing 29; tripartite motif protein TRIM29; tripartite motif-containing 29; Tripartite motif-containing protein 29 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 66 SOURCE: Rabbit