CST, 5184S, Phospho-IRF-7 (Ser471/472) Antibody

Polyclonal Antibody for studying IRF-7 (Ser471/Ser472) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-IRF-7 (Ser471/472) Antibody (CST #5184) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-IRF-7 (Ser471/472) Antibody detects transfected levels of IRF-7 when phosphorylated at Ser471 and 472. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser471/472 of human IRF-7 protein. Antibodies were purified by protein A and peptide affinity chromatography. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response viral infection (6-8). Like IRF-3, IRF-7 is regulated at multiple serine phosphorylation sites near its carboxyl terminus, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11). Alternate Names IMD39; Interferon regulatory factor 7; interferon regulatory factor-7H; IRF-7; IRF-7H; IRF7; IRF7A; IRF7B; IRF7C; IRF7H Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 65 SOURCE: Rabbit