CST, 58009SF, V5-Tag (D3H8Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying . Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, V5-Tag (D3H8Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #58009) is ready to ship. Product Usage Information This product is the carrier free version of product #13202. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13202 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity V5-Tag (D3H8Q) Rabbit Monoclonal Antibody (BSA and Azide Free) detects recombinant proteins containing the V5 epitope tag. This antibody recognizes the V5-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. V5-Tag (D3H8Q) Rabbit Monoclonal Antibody detects exogenously expressed V5-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family (1). The V5 tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems. Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG