Cytiva, 11001302, HiTrap Capto™ Q anion exchange chromatography column

HiTrap Capto Q, 5 × 1 mL HiTrap Capto Q chromatography column is prepacked with a strong anion exchange modern resin for small scale protein purification. • Suitable for screening of selectivity, binding and elution conditions, and for small-scale purifications. • Quaternary ammonium (Q) strong anion exchanger. • Optimized for capture and intermediate protein purification. • Convenient HiTrap format for easy connection to a syringe, peristaltic pump, or chromatography system such as an ÄKTA system. Protein capture and intermediate purification with Capto Q resin Capto Q resin couples a quaternary ammonium (Q) strong anion exchanger to a chemically modified, high-flow agarose matrix. The rigid agarose matrix provides greater particle rigidity without compromising pore size, which allows for high bed volume and purification of viscous samples at high flow rates. High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix. The resin’s high binding-capacity, high flow rate, and low back pressure reduce process cycle times and improve productivity at process scale. Successful use in published research Research applications for this strong anion exchange resin include: The effects of a plant-produced vaccine candidate on Plasmodium falciparum transmission Analysis of outer membrane targeting of secretin PuID protein, and reliance on a dedicated chaperone Molecular characterization of Plasmodium falciparum uracil DNA glycosylase Other Capto Q resin formats The resin in this prepacked column is also available as bulk resin and in other prepacked column formats. Therefore, it is possible to use the same resin for development work, pilot studies, and routine production. Other Cytiva ion exchange products Among Cytiva's solutions are a variety of ion exchange chromatography products, including, Capto, Sepharose, and SOURCE.