Merck, PBGC06210, PES Membrane Filter, 10 kDa NMWCO

Ultrafiltration membranes are typically made from regenerated cellulose or polyethersulfone (PES). The material of choice will greatly depend on sample compatibility. The choice of membrane nominal molecular weight cut-off (NMWCO) will depend on the molecular weight (MW) of the macrosolute that is to be retained. Membrane choice will have a significant impact on performance. As a rule, the NMWCO should be 2-3 times smaller than the molecular weight of the solute to be retained when using regenerated cellulose and 4-5 times smaller for Biomax® PES membranes.,Diafiltration uses ultrafiltration membranes, either in centrifugal or pressure-driven devices such as the Amicon® Stirred Cell for efficient buffer exchange. Unlike dialysis tubing, ultrafiltration membranes can be used for both sample concentration and buffer exchange, thus minimizing sample transfers and reducing sample loss.