Proteintech, 10031-2-AP, Dynamitin (1-83 AA) Polyclonal antibody

Size: 20ul / 150ul The Dynamitin (1-83 AA) (10031-2-AP) by Proteintech is a Polyclonal antibody targeting Dynamitin (1-83 AA) in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 10031-2-AP targets Dynamitin (1-83 AA) in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse testis tissue, mouse lung tissue, human testis tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 59aa of full length p50 dynamitin. Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: dynactin 2 (p50) Calculated Molecular Weight: 50 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC000718 Gene Symbol: Dynamitin Gene ID (NCBI): 10540 RRID: AB_2090613 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13561 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.