Proteintech, 10037-1-Ig, XIAP Polyclonal antibody

Size: 20ul / 150ul The XIAP (10037-1-Ig) by Proteintech is a Polyclonal antibody targeting XIAP in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 10037-1-Ig targets XIAP in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: RAW 264.7 cells, Jurkat cells, HepG2 cells, COLO 320 cells, HeLa cells, C6 cells, NIH/3T3 cells, Raji cells Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0022 Product name: Recombinant human XIAP protein Source: e coli. -derived, Unknow Tag: N/A Domain: XIAP of BC032729 Sequence: Predict reactive species Full Name: X-linked inhibitor of apoptosis Calculated Molecular Weight: 60 kDa Observed Molecular Weight: 45-57 kDa GenBank Accession Number: BC032729 Gene Symbol: XIAP Gene ID (NCBI): 331 RRID: AB_2215009 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P98170 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.