Proteintech, 10088-2-AP, ATG7 Polyclonal antibody

Size: 20ul / 150ul The ATG7 (10088-2-AP) by Proteintech is a Polyclonal antibody targeting ATG7 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 10088-2-AP targets ATG7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue, THP-1 cells, U2OS cells Positive IP detected in: HT-1080 cells, NIH/3T3 cells Positive IHC detected in: human cervical cancer tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, canine, chicken, hamster, goat, turtle, prawn Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0142 Product name: Recombinant human ATG7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 293-563 aa of BC000091 Sequence: AFSPDCPKAVGWEKNQKGGMGPRMVNLSECMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAADRLQKIFPGVNARGFNMSIPMPGHPVNFSSVTLEQARRDVEQLEQLIESHDVVFLLMDTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPNHPVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDST Predict reactive species Full Name: ATG7 autophagy related 7 homolog (S. cerevisiae) Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 68-78 kDa GenBank Accession Number: BC000091 Gene Symbol: ATG7 Gene ID (NCBI): 10533 RRID: AB_2062351 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95352 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.