Proteintech, 10127-2-AP, PCDHA2 Polyclonal antibody

Size: 20ul / 150ul The PCDHA2 (10127-2-AP) by Proteintech is a Polyclonal antibody targeting PCDHA2 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 10127-2-AP targets PCDHA2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, Raji cells, human heart tissue, HeLa cells, human brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue Positive IP detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Protocadherins, which constitute the largest subgroup within the cadherin superfamily, are predominantly expressed in the nervous system and are probably involved in the regulation of neuronal recognition and connectivity (PMID: 17936607; 12231349; 17133224). The protocadherin subfamily can be further subdivided into three groups: the clustered protocadherins, comprising α-, β- and γ-protocadherins; δ-protocadherins; and others, many of which are solitary (PMID: 17133224). PCDHA2 (protocadherin alpha 2) is a single-pass type I membrane protein containing 6 cadherin domains. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. Three isoforms produced by alternative splicing have been described (UniProt). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0173 Product name: Recombinant human PCDHA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 98-259 aa of BC003126 Sequence: RSAECSIHVEVIVDRPLQVFHVEVEVKDINDNPPIFPMTVKTIRFPESRLLDSRFPLEGASDADIGVNALLSYKLSSSEFFFLDIQANDELSESLSLVLGKSLDREETAEVNLLLVATDGGKPELTGTVQILIKVLDVNDNEPTFAQSVYKVKLLENTANGT Predict reactive species Full Name: protocadherin alpha 2 Calculated Molecular Weight: 102 kDa Observed Molecular Weight: 102 kDa, 80 kDa GenBank Accession Number: BC003126 Gene Symbol: PCDHA2 Gene ID (NCBI): 56146 RRID: AB_2158964 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y5H9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.