Proteintech, 10190-2-AP, GNL2 Polyclonal antibody

Size: 20ul / 150ul The GNL2 (10190-2-AP) by Proteintech is a Polyclonal antibody targeting GNL2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 10190-2-AP targets GNL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, K-562 cells, Ramos cells, NIH/3T3 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information GNL2 ( G protein nucleolar 2) has a crucial role in the pathogenesis of gliomas by influencing key cellular processes, particularly enhanced protein synthesis, which ultimately contributes to the development of gliomas. In addition, GNL2 was found to affect the overall protein synthesis of ribosomal protein L11 in glioma cells. The calculated MW of GNL2 is 84 kDa, but the observed MW is around 100 kDa, which may be due to glycosylation modification. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0243 Product name: Recombinant human GNL2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 514-722 aa of BC000107 Sequence: SHCDANTEMQQILTRVRQNFGKINVVPQFSGDDLVPVEVSDLEEELESFSDEEEEEQEQQRDDAEESSSEPEEENVGNDTKAVIKALDEKIAKYQKFLDKAKAKKFSAVRISKGLSEKIFAKPEEQRKTLEEDVDDRAPSKKGKKRKAQREEEQEHSNKAPRALTSKERRRAVRQQRPKKVGVRYYETHNVKNRNRNKKKTNDSEGQKH Predict reactive species Full Name: guanine nucleotide binding protein-like 2 (nucleolar) Calculated Molecular Weight: 731 aa, 84 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC000107 Gene Symbol: GNL2 Gene ID (NCBI): 29889 RRID: AB_2110075 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q13823 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.