Proteintech, 10215-1-AP, FANCG Polyclonal antibody

Size: 20ul / 150ul The FANCG (10215-1-AP) by Proteintech is a Polyclonal antibody targeting FANCG in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 10215-1-AP targets FANCG in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, mouse testis tissue, K-562 cells, HeLa cells, mouse thymus tissue Positive IP detected in: mouse testis tissue Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information FANCG, also named as XRCC9, is a vDNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. It's a candidate tumor suppressor gene. FANCA and FANCG are critical for the localization of the FANC core complex. FANCA and FANCG form a stable complex with a molecular weight of ∼500 kDa that is predicted to be a heterotetramer (PMID: 32002546). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0249 Product name: Recombinant human FANCG protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 125-348 aa of BC000032 Sequence: VLRASCLLPELLSALHRLVGLQAALWLSADRLGDLALLLETLNGSQSGASKDLLLLLKTWSPPAEELDAPLTLQDAQGLKDVLLTAFAYRQGLQELITGNPDKALSSLHEAASGLCPRPVLVQVYTALGSCHRKMGNPQRALLYLVAALKEGSAWGPPLLEASRLYQQLGDTTAELESLELLVEALNVPCSSKAPQFLIEVELLLPPPDLASPLHCGTQSQTKH Predict reactive species Full Name: Fanconi anemia, complementation group G Calculated Molecular Weight: 69 kDa Observed Molecular Weight: 65-68 kDa GenBank Accession Number: BC000032 Gene Symbol: FANCG Gene ID (NCBI): 2189 RRID: AB_2231541 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15287 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.