Proteintech, 10233-1-AP, NDE1 Polyclonal antibody

Size: 20ul / 150ul The NDE1 (10233-1-AP) by Proteintech is a Polyclonal antibody targeting NDE1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 10233-1-AP targets NDE1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A2780 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse ovary tissue, mouse brain tissue, rat brain tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: human breast cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human breast cancer tissue Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0336 Product name: Recombinant human NDE1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 41-282 aa of BC001421 Sequence: EGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQS Predict reactive species Full Name: nudE nuclear distribution gene E homolog 1 (A. nidulans) Calculated Molecular Weight: 335 aa, 38 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC001421 Gene Symbol: NDE1 Gene ID (NCBI): 54820 RRID: AB_2149877 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NXR1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.