Proteintech, 10241-2-AP, CBX1 Polyclonal antibody

Size: 20ul / 150ul The CBX1 (10241-2-AP) by Proteintech is a Polyclonal antibody targeting CBX1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 10241-2-AP targets CBX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A431 cells, MCF-7 cells, HEK-293 cells, human brain tissue, NIH/3T3 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human stomach cancer tissue, mouse testis tissue, human colon cancer tissue, human lung cancer tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0328 Product name: Recombinant human CBX1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 6-185 aa of BC002609 Sequence: NKKKVEEVLEEEEEEYVVEKVLDRRVVKGKVEYLLKWKGFSDEDNTWEPEENLDCPDLIAEFLQSQKTAHETDKSEGGKRKADSDSEDKGEESKPKKKKEESEKPRGFARGLEPERIIGATDSSGELMFLMKWKNSDEADLVPAKEANVKCPQVVISFYEERLTWHSYPSEDDDKKDDKN Predict reactive species Full Name: chromobox homolog 1 (HP1 beta homolog Drosophila ) Calculated Molecular Weight: 27 kDa Observed Molecular Weight: 27 kDa GenBank Accession Number: BC002609 Gene Symbol: CBX1 Gene ID (NCBI): 10951 RRID: AB_2228586 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P83916 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.