Proteintech, 10267-1-AP, MEA1 Polyclonal antibody

Size: 20ul / 150ul The MEA1 (10267-1-AP) by Proteintech is a Polyclonal antibody targeting MEA1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 10267-1-AP targets MEA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, mouse testis tissue Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information MEA1, also named Male-enhanced antigen 1, is a 185 amino acid protein, which is highly expressed in testis. MEA1 may play an important role in spermatogenesis and/or testis development. Endogenous protein was detected as two bands (34 kDa and 36 kDa). (PMID: 12444059) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0375 Product name: Recombinant human MEA1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-185 aa of BC001754 Sequence: MGPERHLSGAPARMATVVLGGDTMGPERIFPNQTEELGHQGPSEGTGDWSSEEPEEEQEETGSGPAGYSYQPLNQDPEQEEVELAPVGDGDVVADIQDRIQALGLHLPDPPLESEDEDEEGATALNNHSSIPMDPEHVELVKRTMAGVSLPAPGVPAWAREISDAQWEDVVQKALQARQASPAWK Predict reactive species Full Name: male-enhanced antigen 1 Calculated Molecular Weight: 20 kDa Observed Molecular Weight: 34 kDa and 36 kDa GenBank Accession Number: BC001754 Gene Symbol: MEA1 Gene ID (NCBI): 4201 RRID: AB_2143838 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16626 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.