Proteintech, 10275-1-AP, SNX17 Polyclonal antibody

Size: 20ul / 150ul The SNX17 (10275-1-AP) by Proteintech is a Polyclonal antibody targeting SNX17 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 10275-1-AP targets SNX17 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells, human brain tissue, mouse skin tissue, mouse brain tissue, mouse cerebellum tissue, RAW 264.7 cells, SK-N-SH cells, SH-SY5Y cells Positive IP detected in: HeLa cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled-coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin and may function in the intracellular trafficking of P-selectin. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0355 Product name: Recombinant human SNX17 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 17-226 aa of BC002524 Sequence: GSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAYDDDVMENRVGLNLLYA Predict reactive species Full Name: sorting nexin 17 Calculated Molecular Weight: 53 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC002524 Gene Symbol: SNX17 Gene ID (NCBI): 9784 RRID: AB_2192394 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15036 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.