Proteintech, 10348-1-AP, STOML2 Polyclonal antibody

Size: 20ul / 150ul The STOML2 (10348-1-AP) by Proteintech is a Polyclonal antibody targeting STOML2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 10348-1-AP targets STOML2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, C6 cells, HEK-293 cells, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human stomach cancer tissue, human endometrial cancer tissue, human breast cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information STOML2 (Stomatin-like protein 2; also known as SLP-2) is a widely expressed mitochondrial member of the highly conserved family of stomatin proteins. STOML2 is localized mostly in mitochondrial inner membrane, minor in plasma membrane. Human STOML2 interacts with prohibitins and regulates mitochondrial biogenesis and function (21746876). In addition, plenty of studies revealed that STOML2 was overexpressed in many human cancer tissues, and its expression may be a new valuable prognostic biomarker (19839737, 21960069). Recently STOML2 has been identified as a serological biomarker for early colorectal cancer (CRC) diagnosis (21209152). Two isoforms of STOML2 exist due to the alternative splicing, with MW of 39 kDa and 33 kDa, respectively. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0363 Product name: Recombinant human STOML2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 82-356 aa of BC002442 Sequence: IVINVPEQSAVTLDNVTLQIDGVLYLRIMDPYKASYGVEDPEYAVTQLAQTTMRSELGKLSLDKVFRERESLNASIVDAINQAADCWGIRCLRYEIKDIHVPPRVKESMQMQVEAERRKRATVLESEGTRESAINVAEGKKQAQILASEAEKAEQINQAAGEASAVLAKAKAKAEAIRILAAALTQHNGDAAASLTVAEQYVSAFSKLAKDSNTILLPSNPGDVTSMVAQAMGVYGALTKAPVPGTPDSLSSGSSRDVQGTDASLDEELDRVKMS Predict reactive species Full Name: stomatin (EPB72)-like 2 Calculated Molecular Weight: 356 aa, 39 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC002442 Gene Symbol: STOML2 Gene ID (NCBI): 30968 RRID: AB_2286822 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UJZ1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.