Proteintech, 10355-1-AP, P21 Polyclonal antibody

Size: 20ul / 150ul The P21 (10355-1-AP) by Proteintech is a Polyclonal antibody targeting P21 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human samples 10355-1-AP targets P21 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HUVEC cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, THP-1 cells, LNCaP cells, NCCIT cells, U2OS cells Positive IP detected in: MCF-7 cells Positive IHC detected in: human colon cancer tissue, human breast cancer tissue, human cervical cancer tissue, human kidney tissue, human stomach cancer tissue, human thyroid cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: myotome of a somite at E11.5 tissue Positive IF/ICC detected in: MCF-7 cells Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:5000-1:20000 Immunofluorescence (IF)-P: IF-P : 1:10-1:100 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported. Specification Tested Reactivity: human Cited Reactivity: human, pig, rabbit, canine, bovine, artemia sinica Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0368 Product name: Recombinant human P21;CDKN1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-149 aa of BC000275 Sequence: MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTD Predict reactive species Full Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1) Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 21 kDa GenBank Accession Number: BC000275 Gene Symbol: p21 Gene ID (NCBI): 1026 RRID: AB_2077682 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P38936 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.