Proteintech, 10406-1-AP, DR1 Polyclonal antibody

Size: 20ul / 150ul The DR1 (10406-1-AP) by Proteintech is a Polyclonal antibody targeting DR1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 10406-1-AP targets DR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse testis tissue, PC-3 cells Positive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:10-1:100 Background Information Down-regulator of transcription 1, TATA box-binding protein (TBP)-binding (negative cofactor 2) is a TBP associated phosphoprotein that represses both basal and activated levels of TBP transcription. DR1 is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. DR1 contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0664 Product name: Recombinant human DR1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-176 aa of BC002809 Sequence: MASSSGNDDDLTIPRAAINKMIKETLPNVRVANDARELVVNCCTEFIHLISSEANEICNKSEKKTISPEHVIQALESLGFGSYISEVKEVLQECKTVALKRRKASSRLENLGIPEEELLRQQQELFAKARQQQAELAQQEWLQMQQAAQQAQLAAASASASNQAGSSQDEEDDDDI Predict reactive species Full Name: down-regulator of transcription 1, TBP-binding (negative cofactor 2) Calculated Molecular Weight: 19 kDa Observed Molecular Weight: 19 kDa GenBank Accession Number: BC002809 Gene Symbol: DR1 Gene ID (NCBI): 1810 RRID: AB_2094676 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q01658 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.