Proteintech, 10525-1-AP, CRMP5 Polyclonal antibody

Size: 20ul / 150ul The CRMP5 (10525-1-AP) by Proteintech is a Polyclonal antibody targeting CRMP5 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 10525-1-AP targets CRMP5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, SH-SY5Y cells, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information DPYSL5(Dihydropyrimidinase-related protein 5) is also named as CRMP5, ULIP6 and belongs to the hydantoinase/dihydropyrimidinase subfamily. It may have a function in neuronal differentiation and/or axon growth. SCLCs universally express CRMP5 and in thymoma, none of the CRMPs are detected by immunohistochemistry in tumorous epithelial cells with specific antibodies including CRMP5.The normal CRMP5 is probably not expressed by tumorous epithelial cells.(PMID:16519949). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0783 Product name: Recombinant human DPYSL5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 265-564 aa of BC002874 Sequence: KVVLAETTTAHATLTGLHYYHQDWSHAAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSGVQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADADVVVWDPEATKTISASTQVQGGDFNLYENMRCHGVPLVTISRGRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRTPYLGDVAVVVHPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPKRASARILAPPGGRSSGIW Predict reactive species Full Name: dihydropyrimidinase-like 5 Calculated Molecular Weight: 61 kDa Observed Molecular Weight: 61 kDa GenBank Accession Number: BC002874 Gene Symbol: CRMP5 Gene ID (NCBI): 56896 RRID: AB_2094644 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BPU6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.