Proteintech, 10583-1-AP, TRAPA/SSR1 Polyclonal antibody

Size: 20ul / 150ul The TRAPA/SSR1 (10583-1-AP) by Proteintech is a Polyclonal antibody targeting TRAPA/SSR1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, monkey samples 10583-1-AP targets TRAPA/SSR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples. Tested Applications Positive WB detected in: COS-7 cells, HepG2 cells, human liver tissue, mouse liver tissue, L02 cells Positive IHC detected in: human ovary tumor tissue, human lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188). Specification Tested Reactivity: human, mouse, monkey Cited Reactivity: human, rat, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0878 Product name: Recombinant human TRAPA, SSR1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC007710 Sequence: MRLLPRLLLLLLLVFPATVLFRGGPRGLLAVAQDLTEDEETVEDSIIEDEDDEAEVEEDEPTDLVEDKEEEDVSGEPEASPSADTTILFVKGEDFPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQDYQFYIQNFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGETIFMYMFLAGLGLLVIVGLHQLLESRKRKRPIQKVEMGTSSQNDVDMSWIPQETLNQINKASPRRLPRKRAQKRSVGSDE Predict reactive species Full Name: signal sequence receptor, alpha Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC007710 Gene Symbol: TRAPA Gene ID (NCBI): 6745 RRID: AB_2195616 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P43307 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.