Proteintech, 10588-1-AP, EXTL3 Polyclonal antibody

Size: 20ul / 150ul The EXTL3 (10588-1-AP) by Proteintech is a Polyclonal antibody targeting EXTL3 in IP, ELISA applications with reactivity to human, mouse samples 10588-1-AP targets EXTL3 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: HEK-293T cells, mouse pancreas tissue Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information Exostosin like glycosyltransferase 3 (EXTL3), also known as RPR, is located at chromosome 8p21.1 and composed of 919 amino acids, making it the longest member of the EXT family (PMID: 35676258). EXTL3 encodes the glycosyltransferases responsible for the biosynthesis of the backbone structure of heparan sulfate (HS) (PMID: 24128412). EXTL3 has been identified as a receptor molecule for regenerating islet-derived (REG) protein ligands, which have been shown to stimulate islet β-cell growth. Mutations in EXTL3 could lead to skeletal dysplasia, developmental delay, and severe immune deficiency (PMID: 28148688). The predicted molecular weight is 105 kDa, but we detected the band at 65 kDa perhaps due to alternative splicing (PMID: 14618397). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0936 Product name: Recombinant human EXTL3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 620-919 aa of BC006363 Sequence: DPVLPSEAKFLGSGTGFRPIGGGAGGSGKEFQAALGGNVPREQFTVVMLTYEREEVLMNSLERLNGLPYLNKVVVVWNSPKLPSEDLLWPDIGVPIMVVRTEKNSLNNRFLPWNEIETEAILSIDDDAHLRHDEIMFGFRVWREARDRIVGFPGRYHAWDIPHQSWLYNSNYSCELSMVLTGAAFFHKYYAYLYSYVMPQAIRDMVDEYINCEDIAMNFLVSHITRKPPIKVTSRWTFRCPGCPQALSHDDSHFHERHKCINFFVKVYGYMPLLYTQFRVDSVLFKTRLPHDKTKCFKFI Predict reactive species Full Name: exostoses (multiple)-like 3 Calculated Molecular Weight: 105 kDa Observed Molecular Weight: 105 kDa GenBank Accession Number: BC006363 Gene Symbol: EXTL3 Gene ID (NCBI): 2137 RRID: AB_2262577 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43909 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.