Proteintech, 10598-1-AP, GORASP2 Polyclonal antibody

Size: 20ul / 150ul The GORASP2 (10598-1-AP) by Proteintech is a Polyclonal antibody targeting GORASP2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 10598-1-AP targets GORASP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells, HeLa cells, rat testis tissue, Raji cells Positive IP detected in: HeLa cells Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, monkey Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0950 Product name: Recombinant human GORASP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 152-452 aa of BC007770 Sequence: LIETHEAKPLKLYVYNTDTDNCREVIITPNSAWGGEGSLGCGIGYGYLHRIPTRPFEEGKKISLPGQMAGTPITPLKDGFTEVQLSSVNPPSLSPPGTTGIEQSLTGLSISSTPPAVSSVLSTGVPTVPLLPPQVNQSLTSVPPMNPATTLPGLMPLPAGLPNLPNLNLNLPAPHIMPGVGLPELVNPGLPPLPSMPPRNLPGIAPLPLPSEFLPSFPLVPESSSAASSGELLSSLPPTSNAPSDPATTTAKADAASSLTVDVTPPTAKAPTTVEDRVGDSTPVSEKPVSAAVDANASESP Predict reactive species Full Name: golgi reassembly stacking protein 2, 55kDa Calculated Molecular Weight: 55 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC007770 Gene Symbol: GORASP2 Gene ID (NCBI): 26003 RRID: AB_2113473 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H8Y8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.