Proteintech, 10602-1-AP, Plexin-B2 Polyclonal antibody

Size: 20ul / 150ul The Plexin-B2 (10602-1-AP) by Proteintech is a Polyclonal antibody targeting Plexin-B2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 10602-1-AP targets Plexin-B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF-7 cells, mouse liver tissue, mouse ovary tissue Positive IP detected in: mouse liver tissue Positive IHC detected in: human tonsillitis tissue, human liver cancer tissue, human breast cancer tissue, human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, chicken, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0941 Product name: Recombinant human PLXNB2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1542-1841 aa of BC004542 Sequence: WKRVNTLMHYNVRDGATLILSKVGVSQQPEDSQQDLPGERHALLEEENRVWHLVRPTDEVDEGKSKRGSVKEKERTKAITEIYLTRLLSVKGTLQQFVDNFFQSVLAPGHAVPPAVKYFFDFLDEQAEKHNIQDEDTIHIWKTNSLPLRFWVNILKNPHFIFDVHVHEVVDASLSVIAQTFMDACTRTEHKLSRDSPSNKLLYAKEISTYKKMVEDYYKGIRQMVQVSDQDMNTHLAEISRAHTDSLNTLVALHQLYQYTQKYYDEIINALEEDPAAQKMQLAFRLQQIAAALENKVTDL Predict reactive species Full Name: plexin B2 Calculated Molecular Weight: 205 kDa Observed Molecular Weight: 80 kDa, 240 kDa GenBank Accession Number: BC004542 Gene Symbol: PLXNB2 Gene ID (NCBI): 23654 RRID: AB_2166424 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15031 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.