Proteintech, 10623-1-AP, FUBP3 Polyclonal antibody

Size: 20ul / 150ul The FUBP3 (10623-1-AP) by Proteintech is a Polyclonal antibody targeting FUBP3 in WB, IP, IHC, ELISA applications with reactivity to human samples 10623-1-AP targets FUBP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information FUBP3, also named as Far upstream element-binding protein 3, is a 572 amino acid protein, which is detected in a number of cell lines and localizes in the nucleus. FUBP3 may interact with single-stranded DNA from the far-upstream element (FUSE) and activate gene expression. FUBP3 exists some isoforms with MV 62 kDa and 28 kDa. Specification Tested Reactivity: human Cited Reactivity: human, pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0974 Product name: Recombinant human FUBP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC007874 Sequence: MPPIGNQLGALVHQRTVITEEFKVPDKMVGFIIGRGGEQISRIQAESGCKIQIASESSGIPERPCVLTGTPESIEQAKRLLGQIVDRCRNGPGFHNDIDSNSTIQEILIPASKVGLVIGRGGETIKQLQERTGVKMVMIQDGPLPTGADKPLRITGDAFKVQQAREMVLEIIREKDQADFRGVRGDFNSRMGGGSIEVSVPRFAVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGISPEYYRQQVAFYGQTLGQAQAHSQEQ Predict reactive species Full Name: far upstream element (FUSE) binding protein 3 Calculated Molecular Weight: 28 kDa Observed Molecular Weight: 62 kDa GenBank Accession Number: BC007874 Gene Symbol: FUBP3 Gene ID (NCBI): 8939 RRID: AB_2104940 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96I24 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.