Proteintech, 10628-1-AP, EEFSEC Polyclonal antibody

Size: 20ul / 150ul The EEFSEC (10628-1-AP) by Proteintech is a Polyclonal antibody targeting EEFSEC in WB, IHC, ELISA applications with reactivity to human samples 10628-1-AP targets EEFSEC in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, A431 cells, HEK-293 cells, Jurkat cells, HepG2 cells Positive IHC detected in: human kidney tissue, human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The amino acid selenocysteine (Sec) is incorporated into a small group of proteins that are involved in preventing cellular oxidative stress. The Sec-specific elongation factor eEFec delivers the aminoacylated selenocysteine-tRNA (Sec-tRNASec) to the ribosome and suppresses UGA codons that are upstream of Sec Insertion Sequence (SECIS) elements bound by SECIS binding protein 2 (SBP2)[PMID:22992746]. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0980 Product name: Recombinant human EEFSEC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 269-569 aa of BC007933 Sequence: KLLERGLVCAPESLHTVHAALISVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPILDSFNFSQEYLFQEQYLSKDLTPAVTDNDEADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADIHTNTCRLAFHGILLHGLEDRNYADSFLPRLKVYKLKHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTGELGIIDSAFGQSGKFKIHIPGGLSPESKKILTPALKKRARAGRGEATRQEESAERSEPSQHVVLSLTFKRYVFDTHKRMVQSP Predict reactive species Full Name: eukaryotic elongation factor, selenocysteine-tRNA-specific Calculated Molecular Weight: 63 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC007933 Gene Symbol: EEFSEC Gene ID (NCBI): 60678 RRID: AB_2293378 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P57772 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.