Proteintech, 10638-1-AP, REDD1 specific Polyclonal antibody

Size: 20ul / 150ul The REDD1 specific (10638-1-AP) by Proteintech is a Polyclonal antibody targeting REDD1 specific in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 10638-1-AP targets REDD1 specific in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A431 cells, DU 145 cells, PC-3 cells, Cobalt Chloride treated HeLa cells, LNCaP cells, K-562 cells, A549 cells, SH-SY5Y cells Positive IP detected in: MCF-7 cells Positive IHC detected in: human lung cancer tissue, human heart tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: LNCaP cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489]. This antibody is a rabbit polyclonal antibody raised against full length human REDD1 antigen. This antibody is specific to the REDD1 from siRNA experiment (PMID:24713927) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, rabbit, squirrel, gerbil Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0965 Product name: Recombinant human REDD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-232 aa of BC007714 Sequence: MPSLWDRFSSSSTSSSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSSNSGFGPEEDTAYLDGVSLPDFELLSDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMPSQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLRLDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLLIEEC Predict reactive species Full Name: DNA-damage-inducible transcript 4 Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 32-35 kDa GenBank Accession Number: BC007714 Gene Symbol: REDD1/DDIT4 Gene ID (NCBI): 54541 RRID: AB_2245711 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NX09 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.