Proteintech, 10725-1-AP, PDCD2 Polyclonal antibody

Size: 20ul / 150ul The PDCD2 (10725-1-AP) by Proteintech is a Polyclonal antibody targeting PDCD2 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, zebrafish samples 10725-1-AP targets PDCD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, zebrafish samples. Tested Applications Positive WB detected in: HeLa cells, H7ES cells, mouse liver tissue Positive IP detected in: HeLa cells Positive IHC detected in: human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Programmed Cell Death 2 (PDCD2) is widely expressed in embryonic and adult tissues but with undefined function yet. Recent observation uncovered its role in hematopoietic stem cell differentiation during development. Catalog# 10725-1-AP is a rabbit polyclonal antibody raised against full-length PDCD2 of human origin. Specification Tested Reactivity: human, mouse, zebrafish Cited Reactivity: human, mouse, rat, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1139 Product name: Recombinant human PDCD2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-344 aa of BC008378 Sequence: MAAAGARPVELGFAESAPAWRLRSEQFPSKVGGRPAWLGAAGLPGPQALACELCGRPLSFLLQVYAPLPGRPDAFHRCIFLFCCREQPCCAGLRVFRNQLPRKNDFYSYEPPSENPPPETGESVCLQLKSGAHLCRVCGCLGPKTCSRCHKAYYCSKEHQTLDWRLGHKQACAQPDHLDHIIPDHNFLFPEFEIVIETEDEIMPEVVEKEDYSEIIGSMGEALEEELDSMAKHESREDKIFQKFKTQIALEPEQILRYGRGIAPIWISGENIPQEKDIPDCPCGAKRILEFQVMPQLLNYLKADRLGKSIDWGILAVFTCAESCSLGTGYTEEFVWKQDVTDTP Predict reactive species Full Name: programmed cell death 2 Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 37-39 kDa, 45 kDa GenBank Accession Number: BC008378 Gene Symbol: PDCD2 Gene ID (NCBI): 5134 RRID: AB_2236895 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16342 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.