Proteintech, 10769-1-AP, Septin 9 Polyclonal antibody

Size: 20ul / 150ul The Septin 9 (10769-1-AP) by Proteintech is a Polyclonal antibody targeting Septin 9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 10769-1-AP targets Septin 9 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: C6 cells, HeLa cells, mouse spleen tissue, mouse brain tissue, mouse thymus tissue, rat brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human pancreas cancer tissue, human colon tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, hTERT-RPE cells, HepG2 cells, A431 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, canine, drosophila Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1218 Product name: Recombinant human SEPT9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 250-568 aa of BC021192 Sequence: RNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGVEEKEPEAPEM Predict reactive species Full Name: septin 9 Calculated Molecular Weight: 64 kDa Observed Molecular Weight: 65 kDa, 38-40 kDa GenBank Accession Number: BC021192 Gene Symbol: SEPT9 Gene ID (NCBI): 10801 RRID: AB_2185012 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UHD8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.