Proteintech, 10770-1-AP, MSI2 Polyclonal antibody

Size: 20ul / 150ul The MSI2 (10770-1-AP) by Proteintech is a Polyclonal antibody targeting MSI2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 10770-1-AP targets MSI2 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PC-12 cells, C6 cells, COLO 320 cells, MCF-7 cells Positive IP detected in: MCF-7 cells Positive IHC detected in: human ovary tumor tissue, human colon cancer tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information MSI2 is an RNA-binding protein that regulates the expression of target mRNAs at the translation level. It binds to RNA via 2 ribonucleoprotein-type RNA recognition motifs in their N-termini, to consensus motifs in mRNAs to inhibit transcription. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. MSI2 can regulate normal hematopoiesis and promote aggressive myeloid leukemia Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1213 Product name: Recombinant human Musashi-2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC001526 Sequence: MEANGSQGTSGSANDSQHDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVTRTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVMFPPGTRGRARGLPYTMDAFMLGMGMLGYPNFVATYGRGYPGFAPSYGYQFPGFPAAAYGPVAAAAVAAARGSGSNPARPGGFPGANSPGPVADLYGPASQDSGVGNYISAASPQPGSGFGHGIAGPLIATAFTNGYH Predict reactive species Full Name: musashi homolog 2 (Drosophila) Calculated Molecular Weight: 35 kDa Observed Molecular Weight: 37-40 kDa GenBank Accession Number: BC001526 Gene Symbol: MSI2 Gene ID (NCBI): 124540 RRID: AB_2147935 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96DH6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.