Proteintech, 10854-1-AP, JP45/JSRP1 Polyclonal antibody

Size: 20ul / 150ul The JP45/JSRP1 (10854-1-AP) by Proteintech is a Polyclonal antibody targeting JP45/JSRP1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 10854-1-AP targets JP45/JSRP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human skeletal muscle tissue Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information JSRP1 (Junctional sarcoplasmic reticulum protein 1), also known as JP-45, is an integral protein of the junctional face membrane of the skeletal muscle sarcoplasmic reticulum (SR), and interacts with calsequestrin and the skeletal-muscle dihydropyridine receptor Cav1. JP-45 is an important protein for functional expression of voltage-dependent calcium channels. Involvement of JP-45 in the molecular mechanism underlying skeletal muscle excitation-contraction coupling has been reported. Although the predicated molecular weight of JP-45 is 36 kDa, it appears a 45-kDa band in western blotting in most cases. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1300 Product name: Recombinant human JP45; JSRP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC021201 Sequence: MSMTTRAWEELDGGLGSCQALEDHSALAETQEDRASATPRLADSGSVPHDSQVAEGPSVDTRPKKMEKEPAARGTPGTGKERLKAGASPRSVPARKKAQTAPPLQPPPPPPALSEELPWGDLSLNKCLVLASLVALLGSAFQLCRDAVPGEAALQARVPEPWVPPSSAPREPSSPLPKFEAQAPPSAPPAPRAEAEVRPKIPGSREAAENDEEEPGEATGEAVREDRVTLADRGPKERPRREGKPRKEKPRKEERPKKERPRKEERPRAAREPREALPQRWESREGGHRPWARDSRDAEPRKKQAWVSPRRPDEEQRPGSRQKLRAGKGRD Predict reactive species Full Name: junctional sarcoplasmic reticulum protein 1 Calculated Molecular Weight: 36 kDa, 45 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC021201 Gene Symbol: JP45/JSRP1 Gene ID (NCBI): 126306 RRID: AB_2129459 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96MG2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.