Proteintech, 10878-1-AP, CD58 Polyclonal antibody

Size: 20ul / 150ul The CD58 (10878-1-AP) by Proteintech is a Polyclonal antibody targeting CD58 in WB, IHC, ELISA applications with reactivity to human samples 10878-1-AP targets CD58 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Raji cells, Jurkat cells, TF-1 cells, HeLa cells, human lymph tissue Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information CD58 a heavily glycosylated protein of 55-70 kDa, is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells. The protein is localized to the plasma membrane. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1165 Product name: Recombinant human CD58 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC005930 Sequence: MVAGSDAGRALGVLSVVCLLHCFGFISCFSQQIYGVVYGNVTFHVPSNVPLKEVLWKKQKDKVAELENSEFRAFSSFKNRVYLDTVSGSLTIYNLTSSDEDEYEMESPNITDTMKFFLYVLESLPSPTLTCALTNGSIEVQCMIPEYYNSHRGLIMYSWDCPMEQCKRNSTSIYFKMENDLPQKIQCTLSNPLFNTTSSIILTTCIPSSGHSRHRYALIPIPLAVITTCIVLYMNGMYAF Predict reactive species Full Name: CD58 molecule Calculated Molecular Weight: 28 kDa Observed Molecular Weight: 55-70 kDa GenBank Accession Number: BC005930 Gene Symbol: CD58 Gene ID (NCBI): 965 RRID: AB_2260179 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P19256 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.