Proteintech, 10910-1-AP, ATP5C1 Polyclonal antibody

Size: 20ul / 150ul The ATP5C1 (10910-1-AP) by Proteintech is a Polyclonal antibody targeting ATP5C1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 10910-1-AP targets ATP5C1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, human heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1343 Product name: Recombinant human ATP5C1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC000931 Sequence: MFSRAGVAGLSAWTLQPQWIQVRNMATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARIYGLGSLALYEKADIKGPEDKKKHLLIGVSSDRGLCGAIHSSIAKQMKSEVATLTAAGKEVMLVGIGDKIRGILYRTHSDQFLVAFKEVGRKPPTFGDASVIALELLNSGYEFDEGSIIFNKFRSVISYKTEEKPIFSLNTVASADSMSIYDDIDADVLQNYQEYNLANIIYYSLKESTTSEQSARMTAMDNASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAALD Predict reactive species Full Name: ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC000931 Gene Symbol: ATP5C1 Gene ID (NCBI): 509 RRID: AB_2877740 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P36542 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.