Proteintech, 10980-1-AP, FceR1a Polyclonal antibody

Size: 20ul / 150ul FceR1a Polyclonal Antibody for WB, ELISA 10980-1-AP targets FceR1a in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MC/9 cells, human spleen tissue, RAW 264.7 cells, mouse spleen tissue, rat spleen tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1398 Product name: Recombinant human FCER1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-257 aa of BC015195 Sequence: MAPAMESPTLLCVALLFFAPDGVLAVPQKPKVSLNPPWNRIFKGENVTLTCNGNNFFEVSSTKWFHNGSLSEETNSSLNIVNAKFEDSGEYKCQHQQVNESEPVYLEVFSDWLLLQASAEVVMEGQPLFLRCHGWRNWDVYKVIYYKDGEALKYWYENHNISITNATVEDSGTYYCTGKVWQLDYESEPLNITVIKAPREKYWLQFFIPLLVVILFAVDTGLFISTQQQATFLLKIKRTRKGFRLLNPHPKPNPKNN Predict reactive species Full Name: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide Calculated Molecular Weight: 30 kDa Observed Molecular Weight: 55-70 kDa GenBank Accession Number: BC015195 Gene Symbol: FceR1a Gene ID (NCBI): 2205 RRID: AB_2103033 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P12319 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.