Proteintech, 11001-1-AP, PANK2 Polyclonal antibody

Size: 20ul / 150ul The PANK2 (11001-1-AP) by Proteintech is a Polyclonal antibody targeting PANK2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11001-1-AP targets PANK2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, human brain tissue, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat brain tissue, rat testis tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human lung cancer tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:10-1:100 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Pantothenate kinase 2 (PANK2) is an essential regulatory enzyme in coenzyme A biosynthesis. PANK2 mutations cause pantothenate kinase-associated neurodegeneration (PKAN), which leads to pigmentary retinopathy, progressive dystonia and other abnormalities(PMID:20603201). PanK2 protein is localized to mitochondria of neurons in human brain, distinguishing it from other pantothenate kinases that do not possess mitochondrial-targeting sequences(PMID:15659606 ). It has 4 isoforms produced by alternative splicing and alternative initiation. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1413 Product name: Recombinant human PANK2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 328-570 aa of BC009421 Sequence: IGDLQLCKLDELDCLIKGILYIDSVGFNGRSQCYYFENPADSEKCQKLPFDLKNPYPLLLVNIGSGVSILAVYSKDNYKRVTGTSLGGGTFFGLCCLLTGCTTFEEALEMASRGDSTKVDKLVRDIYGGDYERFGLPGWAVASSFGNMMSKEKREAVSKEDLARATLITITNNIGSIARMCALNENINQVVFVGNFLRINTIAMRLLAYALDYWSKGQLKALFSEHEGYFGAVGALLELLKIP Predict reactive species Full Name: pantothenate kinase 2 Calculated Molecular Weight: 63 kDa Observed Molecular Weight: 63-70 kDa GenBank Accession Number: BC009421 Gene Symbol: PANK2 Gene ID (NCBI): 80025 RRID: AB_2159041 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BZ23 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.