Proteintech, 11016-1-AP, human IgM Polyclonal antibody

Size: 20ul / 150ul The human IgM (11016-1-AP) by Proteintech is a Polyclonal antibody targeting human IgM in WB, IHC, ELISA applications with reactivity to human samples 11016-1-AP targets human IgM in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human plasma tissue Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:5000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information This antibody detects the heavy chain of human IgM (~75 kDa). Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1459 Product name: Recombinant human IGHM protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC009851 Sequence: FGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLSQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLWATASTFIVLFLLSLFYSTTVTLFKVK Predict reactive species Full Name: immunoglobulin heavy constant mu Calculated Molecular Weight: 69 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC009851 Gene Symbol: IGHM Gene ID (NCBI): 3507 RRID: AB_593462 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.