Proteintech, 11018-1-AP, MUS81 Polyclonal antibody

Size: 20ul / 150ul The MUS81 (11018-1-AP) by Proteintech is a Polyclonal antibody targeting MUS81 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 11018-1-AP targets MUS81 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, NIH/3T3 cells Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information The Crossover junction endonuclease MUS81 has associated endonuclease activity against structure-specific oligonucleotide substrates, including synthetic Holliday junctions(PMID:11741546). It interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The open reading frame of human MUS81 predicts a translation product of 551 amino acids with a molecular mass of 59 kD. There are some reports showed that the enzyme can express a 72 kDa protein in human tissues(PMID:12724407). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1476 Product name: Recombinant human MUS81 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 202-551 aa of BC009999 Sequence: RYSLTPEGLELAQKLAESEGLSLLNVGIGPKEPPGEETAVPGAASAELASEAGVQQQPLELRPGEYRVLLCVDIGETRGGGHRPELLRELQRLHVTHTVRKLHVGDFVWVAQETNPRDPANPGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLERRVYLVEEHGSVHNLSLPESTLLQAVTNTQVIDGFFVKRTADIKESAAYLALLTRGLQRLYQGHTLRSRPWGTPGNPESGAMTSPNPLCSLLTFSDFNAGAIKNKAQSVREVFARQLMQVRGVSGEKAAALVDRYSTPASLLAAYDACATPKEQETLLSTIKCGRLQRNLGPALSRTLSQLYCSYGPLT Predict reactive species Full Name: MUS81 endonuclease homolog (S. cerevisiae) Calculated Molecular Weight: 61 kDa Observed Molecular Weight: 52-72 kDa GenBank Accession Number: BC009999 Gene Symbol: MUS81 Gene ID (NCBI): 80198 RRID: AB_2147130 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96NY9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.