Proteintech, 11038-1-AP, TPM2 Polyclonal antibody

Size: 20ul / 150ul The TPM2 (11038-1-AP) by Proteintech is a Polyclonal antibody targeting TPM2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 11038-1-AP targets TPM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-1080 cells, HeLa cells, mouse skeletal muscle, rat skeletal muscle Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: human breast cancer tissue, human heart tissue, human skeletal muscle tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TPM2, also named as TMSB, belongs to the tropomyosin family. It binds to actin filaments in muscle and non-muscle cells. TPM2 plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. In non-piamuscle cells, TPM2 is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM2 are the cause of nemaline myopathy type 4 (NEM4) and distal arthrogryposis type 1 (DA1). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, rabbit Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1508 Product name: Recombinant human TPM2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-284 aa of BC011776 Sequence: MDAIKKKMQMLKLDKENAIDRAEQAEADKKQAEDRCKQLEEEQQALQKKLKGTEDEVEKYSESVKEAQEKLEQAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMELQEMQLKEAKHIAEDSDRKYEEVARKLVILEGELERSEERAEVAESRARQLEEELRTMDQALKSLMASEEEYSTKEDKYEEEIKLLEEKLKEAETRAEFAERSVAKLEKTIDDLEETLASAKEENVEIHQTLDQTLLELNNL Predict reactive species Full Name: tropomyosin 2 (beta) Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 29-33 kDa GenBank Accession Number: BC011776 Gene Symbol: TPM2 Gene ID (NCBI): 7169 RRID: AB_2205660 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P07951 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.