Proteintech, 11083-1-AP, IFT57 Polyclonal antibody

Size: 20ul / 150ul The IFT57 (11083-1-AP) by Proteintech is a Polyclonal antibody targeting IFT57 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, canine samples 11083-1-AP targets IFT57 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples. Tested Applications Positive WB detected in: HEK-293 cells, mouse retina tissue, NIH/3T3 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human prostate cancer tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: hTERT-RPE1 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information IFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986) Specification Tested Reactivity: human, mouse, rat, canine Cited Reactivity: human, mouse, rat, chicken Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1566 Product name: Recombinant human IFT57 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-429 aa of BC011899 Sequence: MTAALAVVTTSGLEDGVPRSRGEGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPSRHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNATISNILSELRSFGRTADFPPSKLKSGYGEHVCYVLDCFAEEALKYIGFTWKRPIYPVEELEEESVAEDDAELTLNKVDEEFVEEETDNEENFIDLNVLKAQTYHLDMNETAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHRSGIESALKETKGFLDKLHNEITRTLEKISSREKYINNQLENLVQEYRAAQAQLSEAKERYQQGNGGVTERTRLLSEVMEELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVEMDIRIGIVEHTLLQSKLKEKSNMTRNMHATVIPEPATGFY Predict reactive species Full Name: intraflagellar transport 57 homolog (Chlamydomonas) Calculated Molecular Weight: 49 kDa Observed Molecular Weight: 49 kDa GenBank Accession Number: BC011899 Gene Symbol: IFT57 Gene ID (NCBI): 55081 RRID: AB_2121957 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NWB7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.